Monoclonal antibodies (mAbs) are increasingly important in both human and veterinary therapeutics, however porcine specific mAb is limited by both ethical and technical restrictions. Phage display technology is a means to circumvent this problem, as libraries of large diversity can be created without the use of live animals. These libraries can then be screened rapidly to identify high-affinity binders against your pig-specific target. At Creative Biolabs, we have extensive experience in custom pig antibody library construction using phage display. Our scalable platforms can accommodate both naïve and immune libraries and can generate reliable, high affinity antibodies. We offer a full range of phage display library construction services to support your research needs, whether you are looking for monoclonal antibodies for basic research or preclinical studies. We are dedicated to delivering a better, more flexible and cost-effective solution to help you discover highly specific, high-affinity antibodies and speed up your antibody discovery program.
Phage display is a process that genetically links phenotype (displayed protein) with genotype (encoding DNA) by insertion of an exogenous gene sequence in to the genome of a bacteriophage (bacterial virus). The insertion of the gene sequence causes the production of a fusion protein which is displayed on the surface of the phage, while the phage genome's core DNA carries the encoded genetic information for the displayed molecule.
At Creative Biolabs, we harness phage display to accelerate antibody discovery. This powerful technique constructs libraries by genetically linking antibody fragments (scFv or Fab) directly to bacteriophage coat proteins. It allows us to efficiently sift through millions of candidates using iterative biopanning against your specific target antigen, rapidly isolating antibodies with the desired high affinity and specificity. One team has used this technique to develop TGEV-neutralizing antibodies. They built a recombinant scFv phage library from lymphocytes of infected piglets and, through targeted biopanning against TGEV antigen, successfully isolated several high-affinity scFvs that effectively block the virus. This showcases how phage display uniquely enables the rapid generation of functional, species-specific antibodies vital for advancing diagnostics, therapeutic pipelines, and critical research.
At Creative Biolabs, we're your trusted partner for advanced porcine antibody discovery. Our specialized pig antibody construction service harnesses cutting-edge phage display technology, expertly guiding each project to deliver high-fidelity, species-specific antibodies tailored to your research goals. Leveraging meticulously developed porcine antibody libraries, a proven, superior source for both polyclonal and monoclonal candidates, we consistently generate antibodies renowned for their exceptional specificity, robust bioactivity, and superior binding affinity. We ensure excellence with comprehensive services including:
The first step involves selecting and preparing the target antigen. This antigen can be derived from porcine pathogens or specific porcine proteins of interest. The antigen is then purified and immobilized onto a solid support for subsequent screening.
Peripheral blood lymphocytes (PBLs) are isolated from immunized pigs. RNA is extracted from these cells, and complementary DNA (cDNA) encoding the variable regions of the immunoglobulin heavy and light chains is synthesized. These gene fragments are then inserted into phage vectors to create a recombinant phage display library.
The constructed phage library is incubated with the immobilized antigen. Phages displaying antibodies that bind to the antigen are retained, while non-binding phages are washed away. This process is repeated for several rounds to enrich for high-affinity binders.
Individual phage clones are isolated and tested for binding activity. Positive clones are then sequenced to identify the antibody variable regions. These sequences are used to produce recombinant antibodies in suitable expression systems.
Fig.1 Overview of the phage display process.1
Creative Biolabs specializes in providing robust and highly diverse pig antibody libraries constructed through phage display technology, offering numerous advantages for researchers seeking functional antibodies for therapeutic and diagnostic applications. Here's why our pig antibody libraries stand out:
The construction of pig antibody libraries by phage display offers an efficient and scalable method for developing high-affinity antibodies. Creative Biolabs is committed to offering customized phage display services, ensuring that clients receive the most effective and tailored antibody solutions for their research needs. For more information on our pig antibody library construction services, please contact us today!
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Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
