Phage display uses bacteria-infecting viruses (bacteriophages) to make proteins or antibodies appear on their surface. The virus carries the gene for these molecules inside it. This direct link between a molecule's function and its gene code lets scientists quickly test billions of individual viruses to find those that bind strongly to a specific target. At Creative Biolabs, we apply this powerful method to build custom antibody libraries. Our cat antibody library construction service by phage display creates highly specific antibodies that recognize cat-specific targets. We support researchers and veterinarians tackling feline health issues, providing tailored antibody solutions for studies or developing new cat treatments with speed and accuracy.
Creating cat-specific antibodies need special tools. Cats have immune systems work differently from humans or dogs. To fight cat diseases effectively, we need antibodies designed specifically for cats. That's why Creative Biolabs builds cat-focused antibody libraries. These tools help researchers create better tests and treatments for cats. This is important for several reasons:
The most common phage display systems include the M13, T4, and T7 bacteriophages. Each of these systems has its advantages and can be chosen depending on the specific needs of the project.
Fig.1 Phage display systems at Creative Biolabs: M13, T4, and T7 bacteriophages.
Our cat antibody library construction by phage display service is a comprehensive platform designed to streamline the process of generating high-affinity antibodies for feline diseases, pathogens, or biomarkers. Below is an overview of the phage display process, integrated into our service offering:
We begin by obtaining or designing the cat-specific antigen. You can choose to deliver the antigen to Creative Biolabs, or allow Creative Biolabs to make the antigen for you (preferred). By using phage display technology, we can create a comprehensive library based on your specific antigen requirements, ensuring the development of custom monoclonal antibodies that precisely target your feline antigen of interest.
After antigen procurement, we proceed with library screening and biopanning. We identify antibody candidates from pre-built naive libraries. These libraries consist of millions of unique antibodies, ensuring a vast diversity for effective screening. The process includes 4–6 rounds of biopanning, a method that efficiently screens cat antigen-binding phages. This ensures we narrow down our library to the most potent binders.
Fig.2 The process of phage biopanning.1
Once potential binders are identified, we use ELISA screening to further validate individual phage binders. This step ensures that at least 3–10 distinct binders are identified for further analysis. The high specificity of this step ensures that the antibodies are of the highest quality.
Once the optimal binders are identified, we perform DNA extraction and antibody sequencing from inside each phage. This allows us to identify at least three unique binders with high specificity for your cat antigen. We then move on to recombinant antibody production to create the custom antibodies tailored to your research needs.
Using the selected high-affinity binders, we proceed with recombinant antibody production. We provide antibodies in various formats, including monoclonal antibodies, bispecific antibodies, and antibody-drug conjugates (ADCs). We offer solutions that will ensure your project's success from the early-stage discovery through stable cell line development and antibody production.
We are Creative Biolabs, a leader in antibody discovery and phage display services. Here's why you should trust us with your cat antibody library construction. We are able to:
At Creative Biolabs, our cat antibody library construction by phage display service provides you with an unparalleled combination of speed, accuracy, and ethically sourced solutions for feline antibody discovery. With our proven track record, vast experience, and commitment to high-quality service, Creative Biolabs is your trusted partner in custom monoclonal antibody development. Contact us today to learn more about how we can help you achieve your goals with cat antibody library construction.
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Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
