Veterinary immunotherapy is one of the most discussed methods of controlling canine diseases in the last years. One key step to achieve it is to make high-quality and species-specific antibodies. Creative Biolabs provide an advanced phage display library construction service to build an antibody library from dogs by using phage display. Our ultimate goal is to support researchers in discovering and developing monoclonal antibodies for different veterinary applications. This step would save time and money in scientific research.
At Creative Biolabs, we specialize in building large and diverse canine antibody libraries derived from naturally occurring immune responses. Our phage display platform allows for the rapid selection of antibodies that bind specifically to target antigens, ensuring a high degree of specificity and affinity. Our services are tailored to meet the needs of veterinary research, with a focus on antibody development, diagnostic applications, and immunotherapy research for dogs. We offer the following solutions including but not limited to:
We create highly diverse libraries from the synthetic DNA construct, non-immunized or immunized dogs (as illustrated in the table below). This process results in an extensive collection of scFv and Fab fragments, offering a wide range of antibody types for further applications.
| Library Type | Source | Library Size | Diversity | Advantages | Applications |
|---|---|---|---|---|---|
| Immune Library | Immunized dogs | 106 - 108 | Antigen-biased | High affinity to immunogen | Specific antigen targeting |
| Naïve Library | Non-immunized dogs (multiple breeds) | 1010 | Natural repertoire | Broad specificity range | General screening |
| Semi-synthetic Library | Synthetic DNA constructs | 109 - 1011 | Designed diversity | Customizable CDRs (or synthetic & natural CDRs for semi-synthetic library) | Novel epitope discovery |
| Synthetic Library |
Using biopanning, we screen the dog antibody library against antigens of interest, identifying high-affinity antibodies that bind to the target. The process includes ELISA screening and affinity maturation to ensure the best candidates are selected.
Fig.1 The workflow for antibody panning in phage display.1
After identification, we provide detailed characterization of the antibodies, including DNA sequencing, amino acid analysis, and binding affinity assessments, ensuring that the antibodies are suitable for further research.
Our service follows a streamlined, well-established workflow to guarantee the highest quality results:
1. Library Construction
The process begins with the collection of PBMCs from immunized dogs, followed by RNA extraction and cDNA synthesis. The variable regions of the canine heavy and light chains (VH and VL) are then amplified and inserted into the phage display vector.
2. Phage Display and Screening
The phage display library is exposed to the target antigen (such as rabies virus glycoprotein), and biopanning is conducted to select the clones that bind most effectively to the antigen. This step typically involves multiple rounds of selection to increase the stringency and affinity of the clones.
3. Screening and Characterization
Selected phage clones are screened using ELISA and other assays to confirm their binding specificity. Antibodies showing the strongest binding are sequenced to identify their variable regions and to understand their potential for use in research.
4. Antibody Production and Testing
Once the antibody sequences are identified, they are produced as soluble scFv or Fab proteins in E. coli. These antibodies undergo Western blot and neutralization assays to assess their effectiveness in binding and neutralizing the target antigen.
The versatility of dog antibody libraries makes them invaluable for various applications in veterinary research. Some of the key areas of application include:
Dogs get illnesses like cancer, arthritis, and infections. Monoclonal antibody therapies are now helping manage these conditions, offering more precise treatments.
With more and more people embracing therapeutic antibodies in veterinary care, along with clearer regulations and greater investment in pet health, antibodies developed through phage display are becoming a key technology shaping the future of treatments for our canine companions. Creative Biolabs provides cutting-edge services for the development of dog antibody libraries using phage display technology. Our service offers rapid, cost-effective, and highly diverse antibody solutions for scientific research in veterinary medicine, opening new doors for canine-specific antibody development and enabling researchers to make significant strides in veterinary research. Contact Creative Biolabs today to explore how we can assist you in accelerating your veterinary research.
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Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.
