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SS320 Phage Display Cells

Datasheet

CAT:  PDC-SS320
Category:  Phage Display Cells
Specifications
Product Name: SS320 Phage Display Cells
Product Information: SS320 is a non-amber suppressor strain (also known as MC1061F'). Using specific production techniques, our SS320 Phage Display Cells are concentrated stable, can withstand many freeze-thaw cycles without losing titer, and are appropriate for M13 phage work for phage display protein expression.

Applications: These cells are ideal for creating antibody phage display libraries and can also be used for M13 phage work, general cloning, blue/white screening, and antibody fragment scFv, Fab, and VHH expression.
IPTG induction is necessary for optimum scFv, Fab, and VHH expression.
Genotype: [F′proA+B+ lacIqlacZΔM15 Tn10 (tetr)] hsdR mcrB araD139 Δ(araABC-leu)7679 ΔlacX74 galUgalK rpsL thi
Titer: 18 OD600/ml, >2.5×1010 cfu/ml
Doubling Time: ~33 min in 2xYT medium at 37ºC at 250 rpm
Shipping: Ships with Dry Ice
Storage: Store at -80°C.
Competent Cells: 10 x 0.5 ml
Recovery Media: 5 x 2.0 ml

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

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