| CAT: | RP-H-PD1-6His |
| Category: | Recombinant Proteins |
| Specifications | |
| Product Name: | Recombinant Human PD-1 / PDCD1 Protein with C-terminal 6xHis tag |
| Background: | Programmed cell death protein 1 (PD-1), referred to as CD279 and PDCD1, is a type I membrane protein that belongs to the extended CD28/CTLA-4 family of T cell regulators. T cells and pro-B cells express PD-1, a cell surface receptor from the immunoglobulin superfamily. PD-1 interacts two ligands from the B7 family, PD-L1 and PD-L2. IFN-γ therapy induces expression of PD-L1 in practically all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma cells. PD-L2 expression is more restricted, occurring mostly in DCs and a few tumor lines. PD-1 and its ligands inhibit immunological responses. |
| Source: | Human 293 cells (HEK293) |
| UniProt ID: | Q15116 |
| Sequence: | Human PD-1 (Leu 25 - Gln 167) + 6x His tag (N-terminus to C-terminus) |
| Purification: | Affinity and Size-HPLC |
| Purity: | >95% as determined by SDS-PAGE |
| Activity: | Measured by its binding ability (100 ng) to PD-L1 with EC50 =921 ng/ml |
| Endotoxin Level: | <0.10 EU per 1 µg of the antibody by the LAL method |
| Formulation: | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 |
| Reconstitution: | Reconstitute at 0.5 mg/ml in sterile 1xPBS |
| Predicted Molecular Weight: | 18.8 kDa |
| Shipping: | The product is provided in polar packs. Upon receiving, store it immediately at -20 to -70°C |
| Storage: | Steer clear of frequent freeze-thaw cycles and use a manual defrost freezer. • 12 months from the date of receipt at -20 to -70°C, as specified. • 1 month at 2–8°C under sterile circumstances |
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