| CAT: | APDL-11 |
| Category: | Antibody Phage Display Library (Ready-to-Use) |
| Specifications | |
| Product Name: | Ready-to-Use Synthetic VHH Phage Display Library Kit |
| Format: | VHH with HA Tag and 6xHis Tag (pelB leader sequence-VHH-HA tag-6xHis tag) |
| Diversity: | 9.6×10^10 |
| Description: | Framework: ADE99145 Mutated: CDR1, CDR2 and CDR3 (Saturation mutagenesis) NGS was used to check the library's variety and in-frame. The library was built using saturation mutagenesis of all three CDRs and their CDR combinations. The main library is divided into five sub-libraries, each with the following CDR composition and saturation mutant CDR combination. |
| Kit Contents | |
| Component: | Synthetic VHH library (ready-to-panning phage; 8.0×10^12pfu/ml) M13KO7 Helper Phage (2.0×10^12 pfu/ml) Chemically Competent TG1 E. coli (2.5x10^8cfu/ml) M13 Reverse Primer (1.6 µM) pIII Reverse Primer (1.6 µM) |
| Quantity: | 1.0 ml 0.5 ml 0.5 ml 0.2 ml 0.2 ml |
| Composition: | 1×PBS with 25% glycerol 1×PBS with 25% glycerol 2xYT with 25% Glycerol 1×TE Buffer 1×TE Buffer |
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