CAT: | MO-SCFV-PDLC-01 |
Category: | Antibody Phage Display Library |
Specifications> | |
Product Name: | Mouse scFv phage display library construction Kit |
Species: | Mouse |
Product Overview: | Creative Biolabs provides a variety of library primer sets and phagemid vector combinations for antibody and peptide phage display production. Our primer sets and phagemid vectors, with adjustable features and excellent performance, are intended to help you generate phage display libraries in as little as one week. |
Key Features: | High expression efficiency: Engineered for efficient expression and display of antibody fragment scFv on the surface, allowing for easy screening and selection of target molecules. Flexibility and versatility: One vector for both antibody library construction and downstream antibody fragment expression. No need to subclone into an expression vector for downstream application. |
Contents & Storage: | Primer Set 1 for amplification of mouse variable domain of heavy chain (VH) and light chain (Vk and Vλ) Vial 1 200 µl, 10 µM Primer mix (F+R) for Vk repertoires amplification Vial 2 200 µl, 10 µM Primer mix (F+R) for Vλ repertoires amplification Vial 3 200 µl, 10 µM Primer mix (F+R) for VH repertoires amplification Vial 4 200 µl, 10 µM Assembly primers (F+R) for scFv repertoires construction pAPD-m-scFv cloning vector for phage display mouse scFv library construction Vial 5 10.0 µg in Tris-EDTA buffer Sequencing Primer set Via 6 100 µl, 10 µM Forward sequencing primer for mouse scFv insert (M13 Reverse) Via 7 100 µl, 10 µM Reverse sequencing primer for human scFv insert (pIII Reverse) Store at -20°. Vectors are guaranteed stable for 12 months when properly stored. |
Vector usage: | Cloning scFv inserts into pAPD-m-scFv vector for phage display library construction. Cloning scFv candidate into pAPD-m-scFv vector for antibody fragment expression. |
Vector: | pAPD-m-scFv |
Please kindly note that our services can only be used to support research purposes (Not for clinical use).
Creative Biolabs is a globally recognized phage company. Creative Biolabs is committed to providing researchers with the most reliable service and the most competitive price.