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Mouse Fab phage display library construction Kit

Datasheet

CAT:  MO-FAB-PDLC-01
Category:  Antibody Phage Display Library
Specifications
Product Name: Mouse Fab phage display library construction Kit
Species: Mouse
Product Overview: Creative Biolabs provides a variety of library primer sets and phagemid vector combinations for antibody and peptide phage display production. Our primer sets and phagemid vectors, with adjustable features and excellent performance, are intended to help you generate phage display libraries in as little as one week.
Key Features: High expression efficiency: Designed to efficiently express and display antibody fragment on the surface, enabling for simple screening and selection of target molecules.

Flexibility and versatility: One vector for both antibody library construction and downstream antibody fragment expression. There is no need to subclone into an expression vector for downstream applications.
Contents & Storage: Primer Set
Vial 1 100 µl, 10 µM Forward Primer mix (14 oligos) for Vk,λ-Ck,λ fragment amplification
Vial 2 100 µl, 10 µM Reverse Primer mix (2 oligos) for Vk,λ-Ck,λ fragment amplification
Vial 3 100 µl, 10 µM Forward Primer mix (11 oligos) for VH-CH1 fragment amplification
Vial 4 100 µl, 10 µM Reverse Primer mix (3 oligos) for VH-CH1 fragment amplification
Sequencing Primer set
Vial 5 100 µl, 10 µM M13 Reverse primer for scFv forward sequencing
Vial 6 100 µl, 10 µM pIII Reverse primer for scFv reverse sequencing
pAPD-m-Fab vector for mouse Fab phage display library construction
Vial 7 10.0 µg in Tris-EDTA buffer
Store at -20°; Vectors are guaranteed stable for 12 months when properly stored.
Vector usage: Cloning Fab inserts into pAPD-m-Fab vector for phage display library construction.
Cloning Fab candidate into pAPD-m-Fab vector for antibody fragment expression.
Vector: pAPD-m-Fab

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

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