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Chicken scFv phage display library construction Kit

Datasheet

CAT:  CH-SCFV-PDLC-01
Category:  Antibody Phage Display Library
Specifications
Product Name: Chicken scFv phage display library construction Kit
Species: Chicken
Product Overview: Creative Biolabs provides a variety of library primer sets and phagemid vector combinations for antibody and peptide phage display production. Our primer sets and phagemid vectors, with adjustable features and excellent performance, are intended to help you generate phage display libraries in as little as one week.
Key Features: High expression efficiency: Engineered for efficient expression and display of antibody fragment scFv on the surface, allowing for easy screening and selection of target molecules.

Flexibility and versatility: One vector for both antibody library construction and downstream antibody fragment expression. No need to be subcloned into an expression vector for downstream application.
Contents & Storage: Primer for amplification of chicken variable domain of heavy chain (VH) and light chain (VL)
Vial 1 100 µl, 10 µM Primers (normalized forward and reverse primer mix) for VH repertoires amplification
Vial 2 100 µl, 10 µM Primers (normalized forward and reverse primer mix) for VL repertoires amplification
Primer Set for Cloning/assembly chicken scFv (Format: VH-(G4S)3 linker-VL)
Vial 3 100 µl, 10 µM Primers (Forward primer and reverse mix) for chicken scFv repertoires assembly
Sequencing Primer set
Vial 4 100 µl, 10 µM M13 Reverse primer for scFv forward sequencing
Vial 5 100 µl, 10 µM pIII Reverse primer for scFv reverse sequencing
pAPD-ck-scFv cloning vector for phage display chicken scFv library construction
Vial 6 10.0 µg in Tris-EDTA buffer
Store at -20°. Vectors are guaranteed stable for 12 months when properly stored.
Vector usage: Cloning scFv inserts into the pAPD-ck-scFv vector for phage display library construction.
Cloning scFv candidate into pAPD-ck-scFv vector for antibody fragment expression.
Vector: pAPD-ck-scFv

Please kindly note that our services can only be used to support research purposes (Not for clinical use).

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